Jeffrey Bush, PhD

Assistant Professor
Department of Cell & Tissue Biology
+1 415 476-9459

Our lab studies basic mechanisms by which signaling between cells coordinates morphogenesis. Understanding this control has significance beyond its fundamental importance in development since birth defects are the leading cause of death for infants during the first year of life. Craniofacial anomalies are the most common class of congenital defect in humans, with three quarters of all malformations identified at birth involving craniofacial dysmorphogenesis.  We utilize multiple approaches based in mouse genetics to understand fundamental signaling processes as they relate to craniofacial development and disease. In addition to mouse genetics approaches, we utilize human induced pluripotent stem cells and live imaging to understand the cellular and molecular control of morphogenesis.

Research Summary: 
Signaling control of craniofacial development and congenital disease

Websites

Publications: 

EPHRIN-B1 Mosaicism Drives Cell Segregation in Craniofrontonasal Syndrome hiPSC-Derived Neuroepithelial Cells.

Stem cell reports

Niethamer TK, Larson AR, O'Neill AK, Bershteyn M, Hsiao EC, Klein OD, Pomerantz JH, Bush JO

Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation.

The Journal of cell biology

O'Neill AK, Kindberg AA, Niethamer TK, Larson AR, Ho HH, Greenberg ME, Bush JO

Convergence and extrusion are required for normal fusion of the mammalian secondary palate.

PLoS biology

Kim S, Lewis AE, Singh V, Ma X, Adelstein R, Bush JO

Embryonic expression of EphA receptor genes in mice supports their candidacy for involvement in cleft lip and palate.

Developmental dynamics : an official publication of the American Association of Anatomists

Agrawal P, Wang M, Kim S, Lewis AE, Bush JO

The widely used Wnt1-Cre transgene causes developmental phenotypes by ectopic activation of Wnt signaling.

Developmental biology

Lewis AE, Vasudevan HN, O'Neill AK, Soriano P, Bush JO

Ephrin B1 maintains apical adhesion of neural progenitors.

Development (Cambridge, England)

Arvanitis DN, Béhar A, Tryoen-Tóth P, Bush JO, Jungas T, Vitale N, Davy A

Eph/ephrin signaling: genetic, phosphoproteomic, and transcriptomic approaches.

Seminars in cell & developmental biology

Bush JO, Soriano P

The TGF-beta pseudoreceptor gene Bambi is dispensable for mouse embryonic development and postnatal survival.

Genesis (New York, N.Y. : 2000)

Chen J, Bush JO, Ovitt CE, Lan Y, Jiang R

Development of the upper lip: morphogenetic and molecular mechanisms.

Developmental dynamics : an official publication of the American Association of Anatomists

Jiang R, Bush JO, Lidral AC

Expression of Wnt9b and activation of canonical Wnt signaling during midfacial morphogenesis in mice.

Developmental dynamics : an official publication of the American Association of Anatomists

Lan Y, Ryan RC, Zhang Z, Bullard SA, Bush JO, Maltby KM, Lidral AC, Jiang R

The cleft lip and palate defects in Dancer mutant mice result from gain of function of the Tbx10 gene.

Proceedings of the National Academy of Sciences of the United States of America

Bush JO, Lan Y, Jiang R

The T-box gene Tbx10 exhibits a uniquely restricted expression pattern during mouse embryogenesis.

Gene expression patterns : GEP

Bush JO, Maltby KM, Cho ES, Jiang R

Isolation and developmental expression analysis of Tbx22, the mouse homolog of the human X-linked cleft palate gene.

Developmental dynamics : an official publication of the American Association of Anatomists

Bush JO, Lan Y, Maltby KM, Jiang R