Tamara Alliston, PhD

Prof of Orthosurg
Department of Orthopaedic Surgery
allistont@orthosurg.ucsf.edu

Our research focuses on the molecular pathways controlling mesenchymal stem cell differentiation, how these pathways coordinate with physical cues to influence mechanical integrity of normal skeletal tissue, and how they can be harnessed to repair tissue damaged in degenerative skeletal disease. In particular we focus on defining the function of TGFβ in coordinating physical and biochemical cues to regulate skeletal cell differentiation. To answer these questions we combine molecular, cellular, physiologic, and materials science approaches. This interdisciplinary approach will lead to the identification of targets to prevent skeletal disease or to improve skeletal repair.

Research Summary: 
Our research focuses on the molecular pathways controlling mesenchymal stem cell differentiation

Websites

Publications: 

Glucocorticoid suppression of osteocyte perilacunar remodeling is associated with subchondral bone degeneration in osteonecrosis.

Scientific reports

Fowler TW, Acevedo C, Mazur CM, Hall-Glenn F, Fields AJ, Bale HA, Ritchie RO, Lotz JC, Vail TP, Alliston T

Smad4 regulates growth plate matrix production and chondrocyte polarity.

Biology open

Whitaker AT, Berthet E, Cantu A, Laird DJ, Alliston T

Effects of cell type and configuration on anabolic and catabolic activity in 3D co-culture of mesenchymal stem cells and nucleus pulposus cells.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Ouyang A, Cerchiari AE, Tang X, Liebenberg E, Alliston T, Gartner ZJ, Lotz JC

Parallel mechanisms suppress cochlear bone remodeling to protect hearing.

Bone

Jáuregui EJ, Akil O, Acevedo C, Hall-Glenn F, Tsai BS, Bale HA, Liebenberg E, Humphrey MB, Ritchie RO, Lustig LR, Alliston T

Mechanobiology of TGFß signaling in the skeleton.

Matrix biology : journal of the International Society for Matrix Biology

Rys JP, Monteiro DA, Alliston T

Discrete spatial organization of TGFß receptors couples receptor multimerization and signaling to cellular tension.

eLife

Rys JP, DuFort CC, Monteiro DA, Baird MA, Oses-Prieto JA, Chand S, Burlingame AL, Davidson MW, Alliston TN

Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.

NMR in biomedicine

Tufts L, Shet Vishnudas K, Fu E, Kurhanewicz J, Ries M, Alliston T, Li X

Biological regulation of bone quality.

Current osteoporosis reports

Alliston T

Disrupted bone remodeling leads to cochlear overgrowth and hearing loss in a mouse model of fibrous dysplasia.

PloS one

Akil O, Hall-Glenn F, Chang J, Li A, Chang W, Lustig LR, Alliston T, Hsiao EC

Ligand-independent and tissue-selective androgen receptor inhibition by pyrvinium.

ACS chemical biology

Lim M, Otto-Duessel M, He M, Su L, Nguyen D, Chin E, Alliston T, Jones JO

Evolution of a developmental mechanism: Species-specific regulation of the cell cycle and the timing of events during craniofacial osteogenesis.

Developmental biology

Hall J, Jheon AH, Ealba EL, Eames BF, Butcher KD, Mak SS, Ladher R, Alliston T, Schneider RA

Development of a porous poly(DL-lactic acid-co-glycolic acid)-based scaffold for mastoid air-cell regeneration.

The Laryngoscope

Gould TW, Birchall JP, Mallick AS, Alliston T, Lustig LR, Shakesheff KM, Rahman CV

Smad3 binds Scleraxis and Mohawk and regulates tendon matrix organization.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Berthet E, Chen C, Butcher K, Schneider RA, Alliston T, Amirtharajah M

Accumulation of exogenous activated TGF-ß in the superficial zone of articular cartilage.

Biophysical journal

Albro MB, Nims RJ, Cigan AD, Yeroushalmi KJ, Alliston T, Hung CT, Ateshian GA

Regulation of postnatal bone homeostasis by TGFß.

BoneKEy reports

Tang SY, Alliston T

Prolonged alendronate treatment prevents the decline in serum TGF-ß1 levels and reduces cortical bone strength in long-term estrogen deficiency rat model.

Bone

Jia J, Yao W, Amugongo S, Shahnazari M, Dai W, Lay YA, Olvera D, Zimmermann EA, Ritchie RO, Li CS, Alliston T, Lane NE

Matrix metalloproteinase-13 is required for osteocytic perilacunar remodeling and maintains bone fracture resistance.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Tang SY, Herber RP, Ho SP, Alliston T

ECM stiffness primes the TGFß pathway to promote chondrocyte differentiation.

Molecular biology of the cell

Allen JL, Cooke ME, Alliston T

Age-related changes in the plasticity and toughness of human cortical bone at multiple length scales.

Proceedings of the National Academy of Sciences of the United States of America

Zimmermann EA, Schaible E, Bale H, Barth HD, Tang SY, Reichert P, Busse B, Alliston T, Ager JW, Ritchie RO

Structured three-dimensional co-culture of mesenchymal stem cells with chondrocytes promotes chondrogenic differentiation without hypertrophy.

Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society

Cooke ME, Allon AA, Cheng T, Kuo AC, Kim HT, Vail TP, Marcucio RS, Schneider RA, Lotz JC, Alliston T

Local tissue properties of human osteoarthritic cartilage correlate with magnetic resonance T(1) rho relaxation times.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Tang SY, Souza RB, Ries M, Hansma PK, Alliston T, Li X

The use of polyacrylamide gels for mechanical calibration of cartilage--a combined nanoindentation and unconfined compression study.

Journal of the mechanical behavior of biomedical materials

Li C, Allen J, Alliston T, Pruitt LA

Changes in cortical bone response to high-fat diet from adolescence to adulthood in mice.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA

Ionova-Martin SS, Wade JM, Tang S, Shahnazari M, Ager JW, Lane NE, Yao W, Alliston T, Vaisse C, Ritchie RO

Tissue-specific calibration of extracellular matrix material properties by transforming growth factor-ß and Runx2 in bone is required for hearing.

EMBO reports

Chang JL, Brauer DS, Johnson J, Chen CG, Akil O, Balooch G, Humphrey MB, Chin EN, Porter AE, Butcher K, Ritchie RO, Schneider RA, Lalwani A, Derynck R, Marshall GW, Marshall SJ, Lustig L, Alliston T

In situ Materials Characterization using the Tissue Diagnostic Instrument.

Polymer testing

Tang SY, Mathews P, Randall C, Yurtsev E, Fields K, Wong A, Kuo A, Alliston T, Hansma P

Osteopontin deficiency increases bone fragility but preserves bone mass.

Bone

Thurner PJ, Chen CG, Ionova-Martin S, Sun L, Harman A, Porter A, Ager JW, Ritchie RO, Alliston T

Reduced size-independent mechanical properties of cortical bone in high-fat diet-induced obesity.

Bone

Ionova-Martin SS, Do SH, Barth HD, Szadkowska M, Porter AE, Ager JW, Ager JW, Alliston T, Vaisse C, Ritchie RO

The tissue diagnostic instrument.

The Review of scientific instruments

Hansma P, Yu H, Schultz D, Rodriguez A, Yurtsev EA, Orr J, Tang S, Miller J, Wallace J, Zok F, Li C, Souza R, Proctor A, Brimer D, Nogues-Solan X, Mellbovsky L, Peña MJ, Diez-Ferrer O, Mathews P, Randall C, Kuo A, Chen C, Peters M, Kohn D, Buckley J, Li X, Pruitt L, Diez-Perez A, Alliston T, Weaver V, Lotz J

A tense situation: forcing tumour progression.

Nature reviews. Cancer

Butcher DT, Alliston T, Weaver VM

Pharmacologic inhibition of the TGF-beta type I receptor kinase has anabolic and anti-catabolic effects on bone.

PloS one

Mohammad KS, Chen CG, Balooch G, Stebbins E, McKenna CR, Davis H, Niewolna M, Peng XH, Nguyen DH, Ionova-Martin SS, Bracey JW, Hogue WR, Wong DH, Ritchie RO, Suva LJ, Derynck R, Guise TA, Alliston T

Elevated TGF-beta2 signaling in dentin results in sex related enamel defects.

Archives of oral biology

Saeki K, Hilton JF, Alliston T, Habelitz S, Marshall SJ, Marshall GW, Denbesten P

TGF-beta regulation of osteoblast differentiation and bone matrix properties.

Journal of musculoskeletal & neuronal interactions

Alliston T

TGF-beta regulates the mechanical properties and composition of bone matrix.

Proceedings of the National Academy of Sciences of the United States of America

Balooch G, Balooch M, Nalla RK, Schilling S, Filvaroff EH, Marshall GW, Marshall SJ, Ritchie RO, Derynck R, Alliston T

Repression of bone morphogenetic protein and activin-inducible transcription by Evi-1.

The Journal of biological chemistry

Alliston T, Ko TC, Cao Y, Liang YY, Feng XH, Chang C, Derynck R

Medicine: interfering with bone remodelling.

Nature

Alliston T, Derynck R

Molecular mechanisms of ovulation and luteinization.

Molecular and cellular endocrinology

Richards JS, Russell DL, Robker RL, Dajee M, Alliston TN

Ovarian cell differentiation: a cascade of multiple hormones, cellular signals, and regulated genes.

Recent progress in hormone research

Richards JS, Fitzpatrick SL, Clemens JW, Morris JK, Alliston T, Sirois J