Arturo Alvarez-Buylla

Heather and Melanie Muss Professor
Department of Neurological Surgery

Our laboratory studies the mechanisms of adult neurogenesis and neuronal replacement. Contrary to the dogma held for over a century, some populations of neurons continue to be produced in juvenile and adult brains. Basic mechanisms of neural development can be studied in a fully assembled brain, providing key insights into the nature of neural stem cells, mechanisms of neuronal migration, and neural maturation.

Our goals are to understand:

  1. How young neurons are generated.
  2. How young neurons migrate long distances.
  3. How young neurons differentiate and contribute to brain function and possibly brain tumors.
  4. Whether similar processes can be used for brain repair is also studied in the lab.

We have identified the neural stem cells (NSCs), intermediate progenitors cells, and lineages for adult neurogenesis in the adult mammalian ventricular-subventricular zone (V-SVZ). This is the most extensive germinal niche in the adult mammalian brain. These NSCs integrate information within the niche and also receive axonal input from local and distant sources. NSCs in the V-SVZ generate large numbers of new neurons that migrate long distances through the complex matrix of the postnatal brain. New neurons then differentiate into local-circuit neurons that become incorporated into the olfactory bulb. We are studying the properties of adult NSCs, their embryonic origins, and their mode of division.

We are also studying the V-SVZ in the human brain. We found that in infants the V-SVZ contains many migrating young neurons. Interestingly, in addition to the migration to the olfactory bulb (OB) in young children, new neurons derived from the V-SVZ migrate towards cortex. We are investigating: 1) The regions of cortex where these new young neurons migrate to; 2) During which period of human postnatal development they contribute new neurons to cortical circuits; and 3) What types of neurons they generate.

Replacement of local circuit neurons (interneurons) is a promising new approach for brain repair; however, we have found that V-SVZ neurogenesis is highly tailored for the production of olfactory bulb neurons. We are investigating the potential of young neurons derived from the embryonic medial ganglionic eminence (MGE) for brain repair. The MGE is a major source of inhibitory local circuit neurons for the cerebral cortex. Unlike, neurons derived from the adult V-SVZ, young MGE neurons can migrate and functional integrate into the adult brain outside of the olfactory bulb. In collaboration with other laboratories at UCSF, we have found that these cells can induce a new period of plasticity in the visual cortex, can ameliorate seizures in epileptic mice, and can reduce pain thresholds in spinal cord circuits. We are studying how these cells integrate into adult cortex and which populations of interneurons are responsible for the above therapeutic effects.

The laboratory uses mostly in vivo approaches. We take advantage of the collaborative environment at UCSF to use multiple levels of analysis to understand the mechanisms of adult neuronal replacement at the molecular, cellular, circuit, and behavior levels.

Research Summary: 
Birth and Migration of New Neurons

Websites

Publications: 

Transplanted cells are essential for the induction but not the expression of cortical plasticity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hoseini MS, Rakela B, Flores-Ramirez Q, Hasenstaub AR, Alvarez-Buylla A, Stryker MP

Immature excitatory neurons develop during adolescence in the human amygdala.

Nature communications

Sorrells SF, Paredes MF, Velmeshev D, Herranz-Pérez V, Sandoval K, Mayer S, Chang EF, Insausti R, Kriegstein AR, Rubenstein JL, Manuel Garcia-Verdugo J, Huang EJ, Alvarez-Buylla A

Development of Ependymal and Postnatal Neural Stem Cells and Their Origin from a Common Embryonic Progenitor.

Cell reports

Redmond SA, Figueres-Oñate M, Obernier K, Nascimento MA, Parraguez JI, López-Mascaraque L, Fuentealba LC, Alvarez-Buylla A

Neural stem cells: origin, heterogeneity and regulation in the adult mammalian brain.

Development (Cambridge, England)

Obernier K, Alvarez-Buylla A

Multimodal Single-Cell Analysis Reveals Physiological Maturation in the Developing Human Neocortex.

Neuron

Mayer S, Chen J, Velmeshev D, Mayer A, Eze UC, Bhaduri A, Cunha CE, Jung D, Arjun A, Li E, Alvarado B, Wang S, Lovegren N, Gonzales ML, Szpankowski L, Leyrat A, West JAA, Panagiotakos G, Alvarez-Buylla A, Paredes MF, Nowakowski TJ, Pollen AA, Kriegstein AR

Vesicular GABA Transporter Is Necessary for Transplant-Induced Critical Period Plasticity in Mouse Visual Cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Priya R, Rakela B, Kaneko M, Spatazza J, Larimer P, Hoseini MS, Hasenstaub AR, Alvarez-Buylla A, Stryker MP

Does Adult Neurogenesis Persist in the Human Hippocampus?

Cell stem cell

Paredes MF, Sorrells SF, Cebrian-Silla A, Sandoval K, Qi D, Kelley KW, James D, Mayer S, Chang J, Auguste KI, Chang EF, Gutierrez Martin AJ, Kriegstein AR, Mathern GW, Oldham MC, Huang EJ, Garcia-Verdugo JM, Yang Z, Alvarez-Buylla A

A tension-mediated glycocalyx-integrin feedback loop promotes mesenchymal-like glioblastoma.

Nature cell biology

Barnes JM, Kaushik S, Bainer RO, Sa JK, Woods EC, Kai F, Przybyla L, Lee M, Lee HW, Tung JC, Maller O, Barrett AS, Lu KV, Lakins JN, Hansen KC, Obernier K, Alvarez-Buylla A, Bergers G, Phillips JJ, Nam DH, Bertozzi CR, Weaver VM

Transcription Factors Sp8 and Sp9 Coordinately Regulate Olfactory Bulb Interneuron Development.

Cerebral cortex (New York, N.Y. : 1991)

Li J, Wang C, Zhang Z, Wen Y, An L, Liang Q, Xu Z, Wei S, Li W, Guo T, Liu G, Tao G, You Y, Du H, Fu Z, He M, Chen B, Campbell K, Alvarez-Buylla A, Rubenstein JL, Yang Z

Secretagogin is Expressed by Developing Neocortical GABAergic Neurons in Humans but not Mice and Increases Neurite Arbor Size and Complexity.

Cerebral cortex (New York, N.Y. : 1991)

Raju CS, Spatazza J, Stanco A, Larimer P, Sorrells SF, Kelley KW, Nicholas CR, Paredes MF, Lui JH, Hasenstaub AR, Kriegstein AR, Alvarez-Buylla A, Rubenstein JL, Oldham MC

A Glial Signature and Wnt7 Signaling Regulate Glioma-Vascular Interactions and Tumor Microenvironment.

Cancer cell

Griveau A, Seano G, Shelton SJ, Kupp R, Jahangiri A, Obernier K, Krishnan S, Lindberg OR, Yuen TJ, Tien AC, Sabo JK, Wang N, Chen I, Kloepper J, Larrouquere L, Ghosh M, Tirosh I, Huillard E, Alvarez-Buylla A, Oldham MC, Persson AI, Weiss WA, Batchelor TT, Stemmer-Rachamimov A, Suvà ML, Phillips JJ, Aghi MK, Mehta S, Jain RK, Rowitch DH

Human hippocampal neurogenesis drops sharply in children to undetectable levels in adults.

Nature

Sorrells SF, Paredes MF, Cebrian-Silla A, Sandoval K, Qi D, Kelley KW, James D, Mayer S, Chang J, Auguste KI, Chang EF, Gutierrez AJ, Kriegstein AR, Mathern GW, Oldham MC, Huang EJ, Garcia-Verdugo JM, Yang Z, Alvarez-Buylla A

Activity Regulates Cell Death within Cortical Interneurons through a Calcineurin-Dependent Mechanism.

Cell reports

Priya R, Paredes MF, Karayannis T, Yusuf N, Liu X, Jaglin X, Graef I, Alvarez-Buylla A, Fishell G

Adult Neurogenesis Is Sustained by Symmetric Self-Renewal and Differentiation.

Cell stem cell

Obernier K, Cebrian-Silla A, Thomson M, Parraguez JI, Anderson R, Guinto C, Rodas Rodriguez J, Garcia-Verdugo JM, Alvarez-Buylla A

Unique Organization of the Nuclear Envelope in the Post-natal Quiescent Neural Stem Cells.

Stem cell reports

Cebrián-Silla A, Alfaro-Cervelló C, Herranz-Pérez V, Kaneko N, Park DH, Sawamoto K, Alvarez-Buylla A, Lim DA, García-Verdugo JM

Acute Lesioning and Rapid Repair of Hypothalamic Neurons outside the Blood-Brain Barrier.

Cell reports

Yulyaningsih E, Rudenko IA, Valdearcos M, Dahlén E, Vagena E, Chan A, Alvarez-Buylla A, Vaisse C, Koliwad SK, Xu AW

Development and long-term integration of MGE-lineage cortical interneurons in the heterochronic environment.

Journal of neurophysiology

Larimer P, Spatazza J, Stryker MP, Alvarez-Buylla A, Hasenstaub AR

Transplantation of GABAergic interneurons for cell-based therapy.

Progress in brain research

Spatazza J, Mancia Leon WR, Alvarez-Buylla A

Bi- and uniciliated ependymal cells define continuous floor-plate-derived tanycytic territories.

Nature communications

Mirzadeh Z, Kusne Y, Duran-Moreno M, Cabrales E, Gil-Perotin S, Ortiz C, Chen B, Garcia-Verdugo JM, Sanai N, Alvarez-Buylla A

Extensive migration of young neurons into the infant human frontal lobe.

Science (New York, N.Y.)

Paredes MF, James D, Gil-Perotin S, Kim H, Cotter JA, Ng C, Sandoval K, Rowitch DH, Xu D, McQuillen PS, Garcia-Verdugo JM, Huang EJ, Alvarez-Buylla A

Caudal Ganglionic Eminence Precursor Transplants Disperse and Integrate as Lineage-Specific Interneurons but Do Not Induce Cortical Plasticity.

Cell reports

Larimer P, Spatazza J, Espinosa JS, Tang Y, Kaneko M, Hasenstaub AR, Stryker MP, Alvarez-Buylla A

Identification of proliferative progenitors associated with prominent postnatal growth of the pons.

Nature communications

Lindquist RA, Guinto CD, Rodas-Rodriguez JL, Fuentealba LC, Tate MC, Rowitch DH, Alvarez-Buylla A

The Adult Ventricular-Subventricular Zone (V-SVZ) and Olfactory Bulb (OB) Neurogenesis.

Cold Spring Harbor perspectives in biology

Lim DA, Alvarez-Buylla A

Brain size and limits to adult neurogenesis.

The Journal of comparative neurology

Paredes MF, Sorrells SF, Garcia-Verdugo JM, Alvarez-Buylla A

A Dorsal SHH-Dependent Domain in the V-SVZ Produces Large Numbers of Oligodendroglial Lineage Cells in the Postnatal Brain.

Stem cell reports

Tong CK, Fuentealba LC, Shah JK, Lindquist RA, Ihrie RA, Guinto CD, Rodas-Rodriguez JL, Alvarez-Buylla A

Wide Dispersion and Diversity of Clonally Related Inhibitory Interneurons.

Neuron

Harwell CC, Fuentealba LC, Gonzalez-Cerrillo A, Parker PR, Gertz CC, Mazzola E, Garcia MT, Alvarez-Buylla A, Cepko CL, Kriegstein AR

Embryonic Origin of Postnatal Neural Stem Cells.

Cell

Fuentealba LC, Rompani SB, Parraguez JI, Obernier K, Romero R, Cepko CL, Alvarez-Buylla A

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies.

Journal of visualized experiments : JoVE

Vogt D, Wu PR, Sorrells SF, Arnold C, Alvarez-Buylla A, Rubenstein JL

A cortical disinhibitory circuit for enhancing adult plasticity.

eLife

Fu Y, Kaneko M, Tang Y, Alvarez-Buylla A, Stryker MP

Cortical plasticity induced by transplantation of embryonic somatostatin or parvalbumin interneurons.

Proceedings of the National Academy of Sciences of the United States of America

Tang Y, Stryker MP, Alvarez-Buylla A, Espinosa JS

Postnatal growth of the human pons: a morphometric and immunohistochemical analysis.

The Journal of comparative neurology

Tate MC, Lindquist RA, Nguyen T, Sanai N, Barkovich AJ, Huang EJ, Rowitch DH, Alvarez-Buylla A

Astrocyte development and heterogeneity.

Cold Spring Harbor perspectives in biology

Bayraktar OA, Fuentealba LC, Alvarez-Buylla A, Rowitch DH

Adult neural stem cells stake their ground.

Trends in neurosciences

Lim DA, Alvarez-Buylla A

Yoshiki Sasai (1962-2014).

Nature

Alvarez-Buylla A

Primary cilia are required in a unique subpopulation of neural progenitors.

Proceedings of the National Academy of Sciences of the United States of America

Tong CK, Han YG, Shah JK, Obernier K, Guinto CD, Alvarez-Buylla A

Loss of Dishevelleds disrupts planar polarity in ependymal motile cilia and results in hydrocephalus.

Neuron

Ohata S, Nakatani J, Herranz-Pérez V, Cheng J, Belinson H, Inubushi T, Snider WD, García-Verdugo JM, Wynshaw-Boris A, Alvarez-Buylla A

Inhibitory interneuron progenitor transplantation restores normal learning and memory in ApoE4 knock-in mice without or with Aß accumulation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tong LM, Djukic B, Arnold C, Gillespie AK, Yoon SY, Wang MM, Zhang O, Knoferle J, Rubenstein JL, Alvarez-Buylla A, Huang Y

Restricted nature of adult neural stem cells: re-evaluation of their potential for brain repair.

Frontiers in neuroscience

Obernier K, Tong CK, Alvarez-Buylla A

The adult macaque spinal cord central canal zone contains proliferative cells and closely resembles the human.

The Journal of comparative neurology

Alfaro-Cervello C, Cebrian-Silla A, Soriano-Navarro M, Garcia-Tarraga P, Matías-Guiu J, Gomez-Pinedo U, Molina Aguilar P, Alvarez-Buylla A, Luquin MR, Garcia-Verdugo JM

Distinct and separable roles for EZH2 in neurogenic astroglia.

eLife

Hwang WW, Salinas RD, Siu JJ, Kelley KW, Delgado RN, Paredes MF, Alvarez-Buylla A, Oldham MC, Lim DA

Interneurons from embryonic development to cell-based therapy.

Science (New York, N.Y.)

Southwell DG, Nicholas CR, Basbaum AI, Stryker MP, Kriegstein AR, Rubenstein JL, Alvarez-Buylla A

Axonal control of the adult neural stem cell niche.

Cell stem cell

Tong CK, Chen J, Cebrián-Silla A, Mirzadeh Z, Obernier K, Guinto CD, Tecott LH, García-Verdugo JM, Kriegstein A, Alvarez-Buylla A

SnapShot: adult neurogenesis in the V-SVZ.

Neuron

Tong CK, Alvarez-Buylla A

Axons take a dive: Specialized contacts of serotonergic axons with cells in the walls of the lateral ventricles in mice and humans.

Neurogenesis (Austin, Tex.)

Tong CK, Cebrián-Silla A, Paredes MF, Huang EJ, García-Verdugo JM, Alvarez-Buylla A

Molecular diversity subdivides the adult forebrain neural stem cell population.

Stem cells (Dayton, Ohio)

Giachino C, Basak O, Lugert S, Knuckles P, Obernier K, Fiorelli R, Frank S, Raineteau O, Alvarez-Buylla A, Taylor V

Adult neural stem cells in distinct microdomains generate previously unknown interneuron types.

Nature neuroscience

Merkle FT, Fuentealba LC, Sanders TA, Magno L, Kessaris N, Alvarez-Buylla A

Neocortical integration of transplanted GABA progenitor cells from wild type and GABA(B) receptor knockout mouse donors.

Neuroscience letters

Sebe JY, Looke-Stewart E, Dinday MT, Alvarez-Buylla A, Baraban SC

Non-epithelial stem cells and cortical interneuron production in the human ganglionic eminences.

Nature neuroscience

Hansen DV, Lui JH, Flandin P, Yoshikawa K, Rubenstein JL, Alvarez-Buylla A, Kriegstein AR

Myb promotes centriole amplification and later steps of the multiciliogenesis program.

Development (Cambridge, England)

Tan FE, Vladar EK, Ma L, Fuentealba LC, Hoh R, Espinoza FH, Axelrod JD, Alvarez-Buylla A, Stearns T, Kintner C, Krasnow MA

Molecular Characteristics in MRI-Classified Group 1 Glioblastoma Multiforme.

Frontiers in oncology

Haskins WE, Zablotsky BL, Foret MR, Ihrie RA, Alvarez-Buylla A, Eisenman RN, Berger MS, Lin CH

Lineage progression from stem cells to new neurons in the adult brain ventricular-subventricular zone.

Cell cycle (Georgetown, Tex.)

Ponti G, Obernier K, Alvarez-Buylla A

GABA progenitors grafted into the adult epileptic brain control seizures and abnormal behavior.

Nature neuroscience

Hunt RF, Girskis KM, Rubenstein JL, Alvarez-Buylla A, Baraban SC

Functional maturation of hPSC-derived forebrain interneurons requires an extended timeline and mimics human neural development.

Cell stem cell

Nicholas CR, Chen J, Tang Y, Southwell DG, Chalmers N, Vogt D, Arnold CM, Chen YJ, Stanley EG, Elefanty AG, Sasai Y, Alvarez-Buylla A, Rubenstein JL, Kriegstein AR

Use of "MGE enhancers" for labeling and selection of embryonic stem cell-derived medial ganglionic eminence (MGE) progenitors and neurons.

PloS one

Chen YJ, Vogt D, Wang Y, Visel A, Silberberg SN, Nicholas CR, Danjo T, Pollack JL, Pennacchio LA, Anderson S, Sasai Y, Baraban SC, Kriegstein AR, Alvarez-Buylla A, Rubenstein JL

Cell cycle and lineage progression of neural progenitors in the ventricular-subventricular zones of adult mice.

Proceedings of the National Academy of Sciences of the United States of America

Ponti G, Obernier K, Guinto C, Jose L, Bonfanti L, Alvarez-Buylla A

Stem cells in the land of the rising sun: ISSCR 2012.

Cell stem cell

Plath K, Srivastava D, Alvarez-Buylla A, Tanaka EM, Kriegstein AR

Biciliated ependymal cell proliferation contributes to spinal cord growth.

The Journal of comparative neurology

Alfaro-Cervello C, Soriano-Navarro M, Mirzadeh Z, Alvarez-Buylla A, Garcia-Verdugo JM

Intrinsically determined cell death of developing cortical interneurons.

Nature

Southwell DG, Paredes MF, Galvao RP, Jones DL, Froemke RC, Sebe JY, Alfaro-Cervello C, Tang Y, Garcia-Verdugo JM, Rubenstein JL, Baraban SC, Alvarez-Buylla A

Sox1 marks an activated neural stem/progenitor cell in the hippocampus.

Development (Cambridge, England)

Venere M, Han YG, Bell R, Song JS, Alvarez-Buylla A, Blelloch R

Regional astrocyte allocation regulates CNS synaptogenesis and repair.

Science (New York, N.Y.)

Tsai HH, Li H, Fuentealba LC, Molofsky AV, Taveira-Marques R, Zhuang H, Tenney A, Murnen AT, Fancy SP, Merkle F, Kessaris N, Alvarez-Buylla A, Richardson WD, Rowitch DH

Adult neural stem cells bridge their niche.

Cell stem cell

Fuentealba LC, Obernier K, Alvarez-Buylla A

Forebrain GABAergic neuron precursors integrate into adult spinal cord and reduce injury-induced neuropathic pain.

Neuron

Bráz JM, Sharif-Naeini R, Vogt D, Kriegstein A, Alvarez-Buylla A, Rubenstein JL, Basbaum AI

Distinct patterns of human medulloblastoma dissemination in the developing chick embryo nervous system.

Clinical & experimental metastasis

Cage TA, Louie JD, Liu SR, Alvarez-Buylla A, Gupta N, Hyer J

Corridors of migrating neurons in the human brain and their decline during infancy.

Nature

Sanai N, Nguyen T, Ihrie RA, Mirzadeh Z, Tsai HH, Wong M, Gupta N, Berger MS, Huang E, Garcia-Verdugo JM, Rowitch DH, Alvarez-Buylla A

Persistent sonic hedgehog signaling in adult brain determines neural stem cell positional identity.

Neuron

Ihrie RA, Shah JK, Harwell CC, Levine JH, Guinto CD, Lezameta M, Kriegstein AR, Alvarez-Buylla A

Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain.

The Journal of comparative neurology

Sawamoto K, Hirota Y, Alfaro-Cervello C, Soriano-Navarro M, He X, Hayakawa-Yano Y, Yamada M, Hikishima K, Tabata H, Iwanami A, Nakajima K, Toyama Y, Itoh T, Alvarez-Buylla A, Garcia-Verdugo JM, Okano H

Oligodendrogenesis in the subventricular zone and the role of epidermal growth factor.

Brain research reviews

Gonzalez-Perez O, Alvarez-Buylla A

New neurons clear the path of astrocytic processes for their rapid migration in the adult brain.

Neuron

Kaneko N, Marín O, Koike M, Hirota Y, Uchiyama Y, Wu JY, Lu Q, Tessier-Lavigne M, Alvarez-Buylla A, Okano H, Rubenstein JL, Sawamoto K

The subventricular zone en-face: wholemount staining and ependymal flow.

Journal of visualized experiments : JoVE

Mirzadeh Z, Doetsch F, Sawamoto K, Wichterle H, Alvarez-Buylla A

Embryonic MGE precursor cells grafted into adult rat striatum integrate and ameliorate motor symptoms in 6-OHDA-lesioned rats.

Cell stem cell

Martínez-Cerdeño V, Noctor SC, Espinosa A, Ariza J, Parker P, Orasji S, Daadi MM, Bankiewicz K, Alvarez-Buylla A, Kriegstein AR

Cortical plasticity induced by inhibitory neuron transplantation.

Science (New York, N.Y.)

Southwell DG, Froemke RC, Alvarez-Buylla A, Stryker MP, Gandhi SP

Cilia organize ependymal planar polarity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mirzadeh Z, Han YG, Soriano-Navarro M, García-Verdugo JM, Alvarez-Buylla A

Role of primary cilia in brain development and cancer.

Current opinion in neurobiology

Han YG, Alvarez-Buylla A

Cloning mice and men: prohibiting the use of iPS cells for human reproductive cloning.

Cell stem cell

Lo B, Parham L, Alvarez-Buylla A, Cedars M, Conklin B, Fisher S, Gates E, Giudice L, Halme DG, Hershon W, Kriegstein A, Kwok PY, Wagner R

Combinations of genetic mutations in the adult neural stem cell compartment determine brain tumour phenotypes.

The EMBO journal

Jacques TS, Swales A, Brzozowski MJ, Henriquez NV, Linehan JM, Mirzadeh Z, O' Malley C, Naumann H, Alvarez-Buylla A, Brandner S

Reduction of seizures by transplantation of cortical GABAergic interneuron precursors into Kv1.1 mutant mice.

Proceedings of the National Academy of Sciences of the United States of America

Baraban SC, Southwell DG, Estrada RC, Jones DL, Sebe JY, Alfaro-Cervello C, García-Verdugo JM, Rubenstein JL, Alvarez-Buylla A

Dual and opposing roles of primary cilia in medulloblastoma development.

Nature medicine

Han YG, Kim HJ, Dlugosz AA, Ellison DW, Gilbertson RJ, Alvarez-Buylla A

Epidermal growth factor induces the progeny of subventricular zone type B cells to migrate and differentiate into oligodendrocytes.

Stem cells (Dayton, Ohio)

Gonzalez-Perez O, Romero-Rodriguez R, Soriano-Navarro M, Garcia-Verdugo JM, Alvarez-Buylla A

Radiation damage increases Purkinje neuron heterokaryons in neonatal cerebellum.

Annals of neurology

Espejel S, Romero R, Alvarez-Buylla A

Chromatin remodelling factor Mll1 is essential for neurogenesis from postnatal neural stem cells.

Nature

Lim DA, Huang YC, Swigut T, Mirick AL, Garcia-Verdugo JM, Wysocka J, Ernst P, Alvarez-Buylla A

Malignant astrocytomas originate from neural stem/progenitor cells in a somatic tumor suppressor mouse model.

Cancer cell

Alcantara Llaguno S, Chen J, Kwon CH, Jackson EL, Li Y, Burns DK, Alvarez-Buylla A, Parada LF

Identification and characterization of neural progenitor cells in the adult mammalian brain.

Advances in anatomy, embryology, and cell biology

Gil-Perotín S, Alvarez-Buylla A, García-Verdugo JM

The glial nature of embryonic and adult neural stem cells.

Annual review of neuroscience

Kriegstein A, Alvarez-Buylla A

Brain-derived neurotrophic factor signaling does not stimulate subventricular zone neurogenesis in adult mice and rats.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Galvão RP, Garcia-Verdugo JM, Alvarez-Buylla A

The heterogeneity of adult neural stem cells and the emerging complexity of their niche.

Cold Spring Harbor symposia on quantitative biology

Alvarez-Buylla A, Kohwi M, Nguyen TM, Merkle FT

Neural stem cells confer unique pinwheel architecture to the ventricular surface in neurogenic regions of the adult brain.

Cell stem cell

Mirzadeh Z, Merkle FT, Soriano-Navarro M, Garcia-Verdugo JM, Alvarez-Buylla A

Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form Shh-induced medulloblastoma.

Cancer cell

Schüller U, Heine VM, Mao J, Kho AT, Dillon AK, Han YG, Huillard E, Sun T, Ligon AH, Qian Y, Ma Q, Alvarez-Buylla A, McMahon AP, Rowitch DH, Ligon KL

Origin and function of olfactory bulb interneuron diversity.

Trends in neurosciences

Lledo PM, Merkle FT, Alvarez-Buylla A

miR-124 and miR-137 inhibit proliferation of glioblastoma multiforme cells and induce differentiation of brain tumor stem cells.

BMC medicine

Silber J, Lim DA, Petritsch C, Persson AI, Maunakea AK, Yu M, Vandenberg SR, Ginzinger DG, James CD, Costello JF, Bergers G, Weiss WA, Alvarez-Buylla A, Hodgson JG

Primary cilia are required for cerebellar development and Shh-dependent expansion of progenitor pool.

Developmental biology

Spassky N, Han YG, Aguilar A, Strehl L, Besse L, Laclef C, Ros MR, Garcia-Verdugo JM, Alvarez-Buylla A

Hedgehog signaling and primary cilia are required for the formation of adult neural stem cells.

Nature neuroscience

Han YG, Spassky N, Romaguera-Ros M, Garcia-Verdugo JM, Aguilar A, Schneider-Maunoury S, Alvarez-Buylla A

Characterization of adult neural stem cells and their relation to brain tumors.

Cells, tissues, organs

Jackson EL, Alvarez-Buylla A

Developing cell transplantation for temporal lobe epilepsy.

Neurosurgical focus

Richardson RM, Barbaro NM, Alvarez-Buylla A, Baraban SC

Comment on "Human neuroblasts migrate to the olfactory bulb via a lateral ventricular extension".

Science (New York, N.Y.)

Sanai N, Berger MS, Garcia-Verdugo JM, Alvarez-Buylla A

Cells in the astroglial lineage are neural stem cells.

Cell and tissue research

Ihrie RA, Alvarez-Buylla A

Beta-catenin signaling promotes proliferation of progenitor cells in the adult mouse subventricular zone.

Stem cells (Dayton, Ohio)

Adachi K, Mirzadeh Z, Sakaguchi M, Yamashita T, Nikolcheva T, Gotoh Y, Peltz G, Gong L, Kawase T, Alvarez-Buylla A, Okano H, Sawamoto K

Mosaic organization of neural stem cells in the adult brain.

Science (New York, N.Y.)

Merkle FT, Mirzadeh Z, Alvarez-Buylla A

A subpopulation of olfactory bulb GABAergic interneurons is derived from Emx1- and Dlx5/6-expressing progenitors.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kohwi M, Petryniak MA, Long JE, Ekker M, Obata K, Yanagawa Y, Rubenstein JL, Alvarez-Buylla A

Dlx-dependent and -independent regulation of olfactory bulb interneuron differentiation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Long JE, Garel S, Alvarez-Dolado M, Yoshikawa K, Osumi N, Alvarez-Buylla A, Rubenstein JL

The adult neural stem cell niche: lessons for future neural cell replacement strategies.

Neurosurgery clinics of North America

Lim DA, Huang YC, Alvarez-Buylla A

Postnatal deletion of Numb/Numblike reveals repair and remodeling capacity in the subventricular neurogenic niche.

Cell

Kuo CT, Mirzadeh Z, Soriano-Navarro M, Rasin M, Wang D, Shen J, Sestan N, Garcia-Verdugo J, Alvarez-Buylla A, Jan LY, Jan YN

Neural stem cells in mammalian development.

Current opinion in cell biology

Merkle FT, Alvarez-Buylla A

Origin of oligodendrocytes in the subventricular zone of the adult brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Menn B, Garcia-Verdugo JM, Yaschine C, Gonzalez-Perez O, Rowitch D, Alvarez-Buylla A

PDGFR alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling.

Neuron

Jackson EL, Garcia-Verdugo JM, Gil-Perotin S, Roy M, Quinones-Hinojosa A, VandenBerg S, Alvarez-Buylla A

Cortical inhibition modified by embryonic neural precursors grafted into the postnatal brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Alvarez-Dolado M, Calcagnotto ME, Karkar KM, Southwell DG, Jones-Davis DM, Estrada RC, Rubenstein JL, Alvarez-Buylla A, Baraban SC

Composition and organization of the SCZ: a large germinal layer containing neural stem cells in the adult mammalian brain.

Cerebral cortex (New York, N.Y. : 1991)

Seri B, Herrera DG, Gritti A, Ferron S, Collado L, Vescovi A, Garcia-Verdugo JM, Alvarez-Buylla A

Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain.

NeuroImage

Shapiro EM, Gonzalez-Perez O, Manuel García-Verdugo J, Alvarez-Buylla A, Koretsky AP

Olig2-positive progenitors in the embryonic spinal cord give rise not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells.

Developmental biology

Masahira N, Takebayashi H, Ono K, Watanabe K, Ding L, Furusho M, Ogawa Y, Nabeshima Y, Alvarez-Buylla A, Shimizu K, Ikenaka K

Cellular composition and cytoarchitecture of the adult human subventricular zone: a niche of neural stem cells.

The Journal of comparative neurology

Quiñones-Hinojosa A, Sanai N, Soriano-Navarro M, Gonzalez-Perez O, Mirzadeh Z, Gil-Perotin S, Romero-Rodriguez R, Berger MS, Garcia-Verdugo JM, Alvarez-Buylla A

New neurons follow the flow of cerebrospinal fluid in the adult brain.

Science (New York, N.Y.)

Sawamoto K, Wichterle H, Gonzalez-Perez O, Cholfin JA, Yamada M, Spassky N, Murcia NS, Garcia-Verdugo JM, Marin O, Rubenstein JL, Tessier-Lavigne M, Okano H, Alvarez-Buylla A

In vivo transcriptional profile analysis reveals RNA splicing and chromatin remodeling as prominent processes for adult neurogenesis.

Molecular and cellular neurosciences

Lim DA, Suárez-Fariñas M, Naef F, Hacker CR, Menn B, Takebayashi H, Magnasco M, Patil N, Alvarez-Buylla A

Neural stem cells and the origin of gliomas.

The New England journal of medicine

Sanai N, Alvarez-Buylla A, Berger MS

Pax6 is required for making specific subpopulations of granule and periglomerular neurons in the olfactory bulb.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kohwi M, Osumi N, Rubenstein JL, Alvarez-Buylla A

Adult ependymal cells are postmitotic and are derived from radial glial cells during embryogenesis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Spassky N, Merkle FT, Flames N, Tramontin AD, García-Verdugo JM, Alvarez-Buylla A

Sonic hedgehog controls stem cell behavior in the postnatal and adult brain.

Development (Cambridge, England)

Palma V, Lim DA, Dahmane N, Sánchez P, Brionne TC, Herzberg CD, Gitton Y, Carleton A, Alvarez-Buylla A, Ruiz i Altaba A

Radial glia give rise to adult neural stem cells in the subventricular zone.

Proceedings of the National Academy of Sciences of the United States of America

Merkle FT, Tramontin AD, García-Verdugo JM, Alvarez-Buylla A

Cell types, lineage, and architecture of the germinal zone in the adult dentate gyrus.

The Journal of comparative neurology

Seri B, García-Verdugo JM, Collado-Morente L, McEwen BS, Alvarez-Buylla A

Unique astrocyte ribbon in adult human brain contains neural stem cells but lacks chain migration.

Nature

Sanai N, Tramontin AD, Quiñones-Hinojosa A, Barbaro NM, Gupta N, Kunwar S, Lawton MT, McDermott MW, Parsa AT, Manuel-García Verdugo J, Berger MS, Alvarez-Buylla A

Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes.

Nature

Alvarez-Dolado M, Pardal R, Garcia-Verdugo JM, Fike JR, Lee HO, Pfeffer K, Lois C, Morrison SJ, Alvarez-Buylla A

Telomerase activity in the subventricular zone of adult mice.

Molecular and cellular neurosciences

Caporaso GL, Lim DA, Alvarez-Buylla A, Chao MV

Postnatal development of radial glia and the ventricular zone (VZ): a continuum of the neural stem cell compartment.

Cerebral cortex (New York, N.Y. : 1991)

Tramontin AD, García-Verdugo JM, Lim DA, Alvarez-Buylla A

Becoming a new neuron in the adult olfactory bulb.

Nature neuroscience

Carleton A, Petreanu LT, Lansford R, Alvarez-Buylla A, Lledo PM

Permissive corridor and diffusible gradients direct medial ganglionic eminence cell migration to the neocortex.

Proceedings of the National Academy of Sciences of the United States of America

Wichterle H, Alvarez-Dolado M, Erskine L, Alvarez-Buylla A

EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells.

Neuron

Doetsch F, Petreanu L, Caille I, Garcia-Verdugo JM, Alvarez-Buylla A

Maturation and death of adult-born olfactory bulb granule neurons: role of olfaction.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Petreanu L, Alvarez-Buylla A

Neural stem cells and the regulation of neurogenesis in the adult hippocampus.

Clinical neuroscience research

Seri B, Alvarez-Buylla A

Identification of neural stem cells in the adult vertebrate brain.

Brain research bulletin

Alvarez-Buylla A, Seri B, Doetsch F

Lack of the cell-cycle inhibitor p27Kip1 results in selective increase of transit-amplifying cells for adult neurogenesis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Doetsch F, Verdugo JM, Caille I, Alvarez-Buylla A, Chao MV, Casaccia-Bonnefil P

Neurogenesis in adult subventricular zone.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Alvarez-Buylla A, Garcia-Verdugo JM

Multipotent neural stem cells reside into the rostral extension and olfactory bulb of adult rodents.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Gritti A, Bonfanti L, Doetsch F, Caille I, Alvarez-Buylla A, Lim DA, Galli R, Verdugo JM, Herrera DG, Vescovi AL

In utero fate mapping reveals distinct migratory pathways and fates of neurons born in the mammalian basal forebrain.

Development (Cambridge, England)

Wichterle H, Turnbull DH, Nery S, Fishell G, Alvarez-Buylla A

Astrocytes give rise to new neurons in the adult mammalian hippocampus.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Seri B, García-Verdugo JM, McEwen BS, Alvarez-Buylla A

Unsupervised learning and adaptation in a model of adult neurogenesis.

Journal of computational neuroscience

Cecchi GA, Petreanu LT, Alvarez-Buylla A, Magnasco MO

A unified hypothesis on the lineage of neural stem cells.

Nature reviews. Neuroscience

Alvarez-Buylla A, García-Verdugo JM, Tramontin AD

Noggin antagonizes BMP signaling to create a niche for adult neurogenesis.

Neuron

Lim DA, Tramontin AD, Trevejo JM, Herrera DG, García-Verdugo JM, Alvarez-Buylla A

Disruption of Eph/ephrin signaling affects migration and proliferation in the adult subventricular zone.

Nature neuroscience

Conover JC, Doetsch F, Garcia-Verdugo JM, Gale NW, Yancopoulos GD, Alvarez-Buylla A

The subventricular zone: source of neuronal precursors for brain repair.

Progress in brain research

Alvarez-Buylla A, Herrera DG, Wichterle H

Adult-derived neural precursors transplanted into multiple regions in the adult brain.

Annals of neurology

Herrera DG, Garcia-Verdugo JM, Alvarez-Buylla A

Regeneration of a germinal layer in the adult mammalian brain.

Proceedings of the National Academy of Sciences of the United States of America

Doetsch F, García-Verdugo JM, Alvarez-Buylla A

Fate of new neurons in adult canary high vocal center during the first 30 days after their formation.

The Journal of comparative neurology

Kirn JR, Fishman Y, Sasportas K, Alvarez-Buylla A, Nottebohm F

Interaction between astrocytes and adult subventricular zone precursors stimulates neurogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Lim DA, Alvarez-Buylla A

Subventricular zone astrocytes are neural stem cells in the adult mammalian brain.

Cell

Doetsch F, Caillé I, Lim DA, García-Verdugo JM, Alvarez-Buylla A

Young neurons from medial ganglionic eminence disperse in adult and embryonic brain.

Nature neuroscience

Wichterle H, Garcia-Verdugo JM, Herrera DG, Alvarez-Buylla A

Adult subventricular zone neuronal precursors continue to proliferate and migrate in the absence of the olfactory bulb.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kirschenbaum B, Doetsch F, Lois C, Alvarez-Buylla A

Stem cells in the adult mammalian central nervous system.

Current opinion in neurobiology

Temple S, Alvarez-Buylla A

Architecture and cell types of the adult subventricular zone: in search of the stem cells.

Journal of neurobiology

García-Verdugo JM, Doetsch F, Wichterle H, Lim DA, Alvarez-Buylla A

Stem cells in the developing and adult nervous system.

Journal of neurobiology

Alvarez-Buylla A, Temple S

Primary neural precursors and intermitotic nuclear migration in the ventricular zone of adult canaries.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Alvarez-Buylla A, García-Verdugo JM, Mateo AS, Merchant-Larios H

Postnatal mouse subventricular zone neuronal precursors can migrate and differentiate within multiple levels of the developing neuraxis.

Proceedings of the National Academy of Sciences of the United States of America

Lim DA, Fishell GJ, Alvarez-Buylla A

Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Doetsch F, García-Verdugo JM, Alvarez-Buylla A

Direct evidence for homotypic, glia-independent neuronal migration.

Neuron

Wichterle H, Garcia-Verdugo JM, Alvarez-Buylla A

Mechanism of migration of olfactory bulb interneurons.

Seminars in cell & developmental biology

Alvarez-Buylla A

Neurogenesis in juvenile and adult ring doves.

The Journal of comparative neurology

Ling C, Zuo M, Alvarez-Buylla A, Cheng MF

Network of tangential pathways for neuronal migration in adult mammalian brain.

Proceedings of the National Academy of Sciences of the United States of America

Doetsch F, Alvarez-Buylla A

Adenovirus-mediated gene delivery into neuronal precursors of the adult mouse brain.

Proceedings of the National Academy of Sciences of the United States of America

Yoon SO, Lois C, Alvirez M, Alvarez-Buylla A, Falck-Pedersen E, Chao MV

Chain migration of neuronal precursors.

Science (New York, N.Y.)

Lois C, García-Verdugo JM, Alvarez-Buylla A

Neuronal stem cells in the brain of adult vertebrates.

Stem cells (Dayton, Ohio)

Alvarez-Buylla A, Lois C

Testosterone increases the recruitment and/or survival of new high vocal center neurons in adult female canaries.

Proceedings of the National Academy of Sciences of the United States of America

Rasika S, Nottebohm F, Alvarez-Buylla A

The life span of new neurons in a song control nucleus of the adult canary brain depends on time of year when these cells are born.

Proceedings of the National Academy of Sciences of the United States of America

Nottebohm F, O'Loughlin B, Gould K, Yohay K, Alvarez-Buylla A

Long-distance neuronal migration in the adult mammalian brain.

Science (New York, N.Y.)

Lois C, Alvarez-Buylla A

Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia.

Proceedings of the National Academy of Sciences of the United States of America

Lois C, Alvarez-Buylla A

Neurogenesis and plasticity in the CNS of adult birds.

Experimental neurology

Alvarez-Buylla A

Production and survival of projection neurons in a forebrain vocal center of adult male canaries.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kirn JR, Alvarez-Buylla A, Nottebohm F

On variables that affect estimates of the true sizes and densities of radioactively labeled cell nuclei.

The Journal of comparative neurology

Clark SJ, Cynx J, Alvarez-Buylla A, O'Loughlin B, Nottebohm F

Birth of projection neurons in adult avian brain may be related to perceptual or motor learning.

Science (New York, N.Y.)

Alvarez-Buylla A, Kirn JR, Nottebohm F

Song learning in birds: the relation between perception and production.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

Nottebohm F, Alvarez-Buylla A, Cynx J, Kirn J, Ling CY, Nottebohm M, Suter R, Tolles A, Williams H

Cresyl violet: a red fluorescent Nissl stain.

Journal of neuroscience methods

Alvarez-Buylla A, Ling CY, Kirn JR

Expression of the yes proto-oncogene in cerebellar Purkinje cells.

Molecular and cellular biology

Sudol M, Kuo CF, Shigemitsu L, Alvarez-Buylla A

In situ hybridization using PEG-embedded tissue and riboprobes: increased cellular detail coupled with high sensitivity.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Clayton DF, Alvarez-Buylla A

Birth of projection neurons in the higher vocal center of the canary forebrain before, during, and after song learning.

Proceedings of the National Academy of Sciences of the United States of America

Alvarez-Buylla A, Theelen M, Nottebohm F

Migration of young neurons in adult avian brain.

Nature

Alvarez-Buylla A, Nottebohm F

Simple microcomputer system for mapping tissue sections with the light microscope.

Journal of neuroscience methods

Alvarez-Buylla A, Vicario DS

Mapping of radial glia and of a new cell type in adult canary brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Alvarez-Buylla A, Theelen M, Nottebohm F

Monoclonal antibody reveals radial glia in adult avian brain.

The Journal of comparative neurology

Alvarez-Buylla A, Buskirk DR, Nottebohm F

Characterization of alkaline phosphatase from primordial germ cells and ontogenesis of this enzyme in the mouse.

Differentiation; research in biological diversity

Merchant-Larios H, Mendlovic F, Alvarez-Buylla A