Martin Kampmann, PhD

Associate Professor
Biochemistry and Biophysics
[email protected]
+1 415 514-5545

The Kampmann lab develops and applies innovative technologies to understand cellular and molecular mechanisms of aging-associated neurodegenerative diseases, and to discover new therapeutic strategies. A major focus of our research are Alzheimer's disease and related dementias.

We have pioneered a CRISPR-based functional genomics platform in human iPSC-derived neurons, glia and 3D assembloids, which enables genome-wide modifier screens of disease-relevant cell biology in patient-derived cells. We have also developed cell-type specific CRISPR screening approaches for mouse models of disease.

We use biochemistry, biophysics and cell biology to test mechanistic hypotheses generated by our functional genomics platform.

Major research questions are:

What controls protein aggregation in neurons, and why is it toxic? Why are some neuronal subtypes selectively vulnerable? Our main focus here is on the protein tau.

How do non-neuronal cells such as microglia and astrocytes contribute to disease? What controls their beneficial and toxic states and functions in disease?

Research Summary: 
We develop and apply innovative approaches, such as CRISPR-based functional genomics, in human iPSC-derived neurons and glia and mouse models to elucidate mechanisms and therapeutic strategies for neurodegenerative diseases.
Publications: 

Human iPSC 4R tauopathy model uncovers modifiers of tau propagation.

Cell

Parra Bravo C, Giani AM, Perez JM, Zhao Z, Wan Y, Samelson AJ, Wong MY, Evangelisti A, Cordes E, Fan L, Ye P, Zhu D, Pozner T, Mercedes M, Patel T, Yarahmady A, Carling GK, Sterky FH, Lee VMY, Lee EB, DeTure M, Dickson DW, Sharma M, Mok SA, Luo W, Zhao M, Kampmann M, Gong S, Gan L

International consensus guidelines for the definition, detection, and interpretation of autophagy-dependent ferroptosis.

Autophagy

Chen X, Tsvetkov AS, Shen HM, Isidoro C, Ktistakis NT, Linkermann A, Koopman WJH, Simon HU, Galluzzi L, Luo S, Xu D, Gu W, Peulen O, Cai Q, Rubinsztein DC, Chi JT, Zhang DD, Li C, Toyokuni S, Liu J, Roh JL, Dai E, Juhasz G, Liu W, Zhang J, Yang M, Liu J, Zhu LQ, Zou W, Piacentini M, Ding WX, Yue Z, Xie Y, Petersen M, Gewirtz DA, Mandell MA, Chu CT, Sinha D, Eftekharpour E, Zhivotovsky B, Besteiro S, Gabrilovich DI, Kim DH, Kagan VE, Bayir H, Chen GC, Ayton S, Lünemann JD, Komatsu M, Krautwald S, Loos B, Baehrecke EH, Wang J, Lane JD, Sadoshima J, Yang WS, Gao M, Münz C, Thumm M, Kampmann M, Yu D, Lipinski MM, Jones JW, Jiang X, Zeh HJ, Kang R, Klionsky DJ, Kroemer G, Tang D

Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesis.

bioRxiv : the preprint server for biology

Sun N, Teyssier N, Wang B, Drake S, Seyler M, Zaltsman Y, Everitt A, Teerikorpi N, Willsey HR, Goodarzi H, Tian R, Kampmann M, Willsey AJ

Alzheimer’s disease risk genes modulate interferon-responsive microglia.

Alzheimer's & Dementia

Amanda McQuade, Vincent Cele Castillo, Nina Drager, Martin Kampmann

Multiomics of iPSC-derived ApoE3- and ApoE4 astrocytes and microglia uncovers cell type specific dysregulation of immunometabolism.

Alzheimer's & Dementia

Rik van der Kant, Niek Blomberg, Lena Erlebach, Femke M Feringa, Jorin Heijneman, Sascha Koppes, Iris Daphne Kruijff, Amanda McQuade, Ricardo Miramontes, Damien Olivier-Jimenez, Aiko Robert, Leslie M. Thompson, Alessandro Ori, Deborah Kronenberg-Versteeg, Yassene Mohammed, Martin Kampmann, Martin Giera

Phospholipase D3 drives microglial response to amyloid pathology in Alzheimer’s disease.

Alzheimer's & Dementia

Matthew J Rosene, Emma Danhash, Shih-Feng You, Abhirami Kannan Iyer, Simon Hsu, Mariana Acquarone de Sa Lopes, Logan Brase, Anthony Verbeck, Rita Martinez, Clare E Wallace, Zeran Li, Ping Yan, Nina Drager, Sydney M Sattler, Thomas E Mahan, Shannon L Macauley, David M. Holtzman, Bruno A. Benitez, Martin Kampmann, Carlos Cruchaga, Oscar Harari, John R Cirrito, Jin-Moo Lee, Alison Goate, Celeste M. Karch

Pharmacologic activation of a compensatory integrated stress response kinase promotes mitochondrial remodeling in PERK-deficient cells.

Cell chemical biology

Perea V, Baron KR, Dolina V, Aviles G, Kim G, Rosarda JD, Guo X, Kampmann M, Wiseman RL

Neurodegeneration enters the era of functional genomics.

Science (New York, N.Y.)

Aguzzi A, Kampmann M

Tau fibrils induce nanoscale membrane damage and nucleate cytosolic tau at lysosomes.

bioRxiv : the preprint server for biology

Rose K, Jepson T, Shukla S, Maya-Romero A, Kampmann M, Xu K, Hurley JH

DELE1 oligomerization promotes integrated stress response activation.

Nature structural & molecular biology

Yang J, Baron KR, Pride DE, Schneemann A, Guo X, Chen W, Song AS, Aviles G, Kampmann M, Wiseman RL, Lander GC

Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation.

Developmental cell

Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, Kampmann M, Kopito RR, Faghri F, Olzmann JA

Scalable, cell type-selective, AAV-based in vivo CRISPR screening in the mouse brain.

bioRxiv : the preprint server for biology

Ramani B, Rose IVL, Pan A, Tian R, Ma K, Palop JJ, Kampmann M

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.

bioRxiv : the preprint server for biology

Samelson AJ, Ariqat N, McKetney J, Rohanitazangi G, Bravo CP, Goodness D, Tian R, Grosjean P, Abskharon R, Eisenberg D, Kanaan NM, Gan L, Condello C, Swaney DL, Kampmann M

Human iPSC 4R tauopathy model uncovers modifiers of tau propagation.

bioRxiv : the preprint server for biology

Bravo CP, Giani AM, Perez JM, Zhao Z, Samelson A, Wong MY, Evangelisti A, Fan L, Pozner T, Mercedes M, Ye P, Patel T, Yarahmady A, Carling G, Lee VMY, Sharma M, Mok SA, Luo W, Zhao M, Kampmann M, Gong S, Gan L

Pharmacologic Activation of a Compensatory Integrated Stress Response Kinase Promotes Mitochondrial Remodeling in PERK-deficient Cells.

bioRxiv : the preprint server for biology

Perea V, Baron KR, Dolina V, Aviles G, Rosarda JD, Guo X, Kampmann M, Wiseman RL

Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles.

Nature communications

Lan Y, van Leur SW, Fernando JA, Wong HH, Kampmann M, Siu L, Zhang J, Li M, Nicholls JM, Sanyal S

CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions.

bioRxiv : the preprint server for biology

Li E, Benitez C, Boggess SC, Koontz M, Rose IVL, Draeger N, Teter OM, Samelson AJ, Ullian EM, Kampmann M

Neurons require glucose uptake and glycolysis in vivo.

Cell reports

Li H, Guglielmetti C, Sei YJ, Zilberter M, Le Page LM, Shields L, Yang J, Nguyen K, Tiret B, Gao X, Bennett N, Lo I, Dayton TL, Kampmann M, Huang Y, Rathmell JC, Vander Heiden M, Chaumeil MM, Nakamura K

A molecular glue approach to control the half-life of CRISPR-based technologies.

bioRxiv : the preprint server for biology

Sreekanth V, Jan M, Zhao KT, Lim D, Davis JR, McConkey M, Kovalcik V, Barkal S, Law BK, Fife J, Tian R, Vinyard ME, Becerra B, Kampmann M, Sherwood RI, Pinello L, Liu DR, Ebert BL, Choudhary A

Granulin loss of function in human mature brain organoids implicates astrocytes in TDP-43 pathology.

Stem cell reports

de Majo M, Koontz M, Marsan E, Salinas N, Ramsey A, Kuo YM, Seo K, Li H, Dr?ger N, Leng K, Gonzales SL, Kurnellas M, Miyaoka Y, Klim JR, Kampmann M, Ward ME, Huang EJ, Ullian EM

Genomic profiling of HIV-1 integration in microglia cells links viral integration to the topologically associated domains.

Cell reports

Rheinberger M, Costa AL, Kampmann M, Glavas D, Shytaj IL, Sreeram S, Penzo C, Tibroni N, Garcia-Mesa Y, Leskov K, Fackler OT, Vlahovicek K, Karn J, Lucic B, Herrmann C, Lusic M

MS4A4A modifies the risk of Alzheimer disease by regulating lipid metabolism and immune response in a unique microglia state.

medRxiv : the preprint server for health sciences

You SF, Brase L, Filipello F, Iyer AK, Del-Aguila J, He J, D'Oliveira Albanus R, Budde J, Norton J, Gentsch J, Dräger NM, Sattler SM, Kampmann M, Piccio L, Morris JC, Perrin RJ, McDade E, Dominantly Inherited Alzheimer Network, Paul SM, Cashikar AG, Benitez BA, Harari O, Karch CM

An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes.

Cell

Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J

Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors.

eLife

Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M

Pathological Validation of RORB and CDH9 as Selectively Vulnerable Neurons in Alzheimer’s Disease.

Alzheimer's & Dementia

Rakin T Nasar, Felipe Luiz Pereira, Song Hua Li, Silvia Miramontes, Martin Kampmann, Kun Leng, William W. Seeley, Salvatore Spina, Bruce L. Miller, Lea Tenenholz Grinberg

A reference human induced pluripotent stem cell line for large-scale collaborative studies.

Cell stem cell

Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, Santiana M, Faghri F, Nalls MA, Vitale D, Ballard S, Qi YA, Ramos DM, Anderson KM, Stadler J, Narayan P, Papademetriou J, Reilly L, Nelson MP, Aggarwal S, Rosen LU, Kirwan P, Pisupati V, Coon SL, Scholz SW, Priebe T, ?ttl M, Dong J, Meijer M, Janssen LJM, Lourenco VS, van der Kant R, Crusius D, Paquet D, Raulin AC, Bu G, Held A, Wainger BJ, Gabriele RMC, Casey JM, Wray S, Abu-Bonsrah D, Parish CL, Beccari MS, Cleveland DW, Li E, Rose IVL, Kampmann M, Calatayud Aristoy C, Verstreken P, Heinrich L, Chen MY, Sch?le B, Dou D, Holzbaur ELF, Zanellati MC, Basundra R, Deshmukh M, Cohen S, Khanna R, Raman M, Nevin ZS, Matia M, Van Lent J, Timmerman V, Conklin BR, Johnson Chase K, Zhang K, Funes S, Bosco DA, Erlebach L, Welzer M, Kronenberg-Versteeg D, Lyu G, Arenas E, Coccia E, Sarrafha L, Ahfeldt T, Marioni JC, Skarnes WC, Cookson MR, Ward ME, Merkle FT

Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin.

Nature communications

Kim H, Leng K, Park J, Sorets AG, Kim S, Shostak A, Embalabala RJ, Mlouk K, Katdare KA, Rose IVL, Sturgeon SM, Neal EH, Ao Y, Wang S, Sofroniew MV, Brunger JM, McMahon DG, Schrag MS, Kampmann M, Lippmann ES

CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states.

Nature neuroscience

Leng K, Rose IVL, Kim H, Xia W, Romero-Fernandez W, Rooney B, Koontz M, Li E, Ao Y, Wang S, Krawczyk M, Tcw J, Goate A, Zhang Y, Ullian EM, Sofroniew MV, Fancy SPJ, Schrag MS, Lippmann ES, Kampmann M

Reduced endosomal microautophagy activity in aging associates with enhanced exocyst-mediated protein secretion.

Aging cell

Krause GJ, Diaz A, Jafari M, Khawaja RR, Agullo-Pascual E, Santiago-Fernández O, Richards AL, Chen KH, Dmitriev P, Sun Y, See SK, Abdelmohsen K, Mazan-Mamczarz K, Krogan NJ, Gorospe M, Swaney DL, Sidoli S, Bravo-Cordero JJ, Kampmann M, Cuervo AM

Functional regulatory variants implicate distinct transcriptional networks in dementia.

Science (New York, N.Y.)

Cooper YA, Teyssier N, Dräger NM, Guo Q, Davis JE, Sattler SM, Yang Z, Patel A, Wu S, Kosuri S, Coppola G, Kampmann M, Geschwind DH

A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states.

Nature neuroscience

Dräger NM, Sattler SM, Huang CT, Teter OM, Leng K, Hashemi SH, Hong J, Aviles G, Clelland CD, Zhan L, Udeochu JC, Kodama L, Singleton AB, Nalls MA, Ichida J, Ward ME, Faghri F, Gan L, Kampmann M

Phenotypic Screening Using High-Content Imaging to Identify Lysosomal pH Modulators in a Neuronal Cell Model.

ACS chemical neuroscience

Chin MY, Ang KH, Davies J, Alquezar C, Garda VG, Rooney B, Leng K, Kampmann M, Arkin MR, Kao AW

Conversations with LGBT+ scientists about visibility, leadership and climbing the career ladder.

Journal of cell science

Bristow RG, Engel J, Jayasinghe I, Kampmann M, James Sansom O, Bryant DM

Selective vulnerabilities in the proteostasis network of castration-resistant prostate cancer.

Cell chemical biology

Shkedi A, Taylor IR, Echtenkamp F, Ramkumar P, Alshalalfa M, Rivera-Márquez GM, Moses MA, Shao H, Karnes RJ, Neckers L, Feng F, Kampmann M, Gestwicki JE

NudC guides client transfer between the Hsp40/70 and Hsp90 chaperone systems.

Molecular cell

Biebl MM, Delhommel F, Faust O, Zak KM, Agam G, Guo X, Mühlhofer M, Dahiya V, Hillebrand D, Popowicz GM, Kampmann M, Lamb DC, Rosenzweig R, Sattler M, Buchner J

BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2.

Nature cell biology

Samelson AJ, Tran QD, Robinot R, Carrau L, Rezelj VV, Kain AM, Chen M, Ramadoss GN, Guo X, Lim SA, Lui I, Nuñez JK, Rockwood SJ, Wang J, Liu N, Carlson-Stevermer J, Oki J, Maures T, Holden K, Weissman JS, Wells JA, Conklin BR, TenOever BR, Chakrabarti LA, Vignuzzi M, Tian R, Kampmann M

CRISPR-Based Screening for Stress Response Factors in Mammalian Cells.

Methods in molecular biology (Clifton, N.J.)

Guo X, Kampmann M

Evaluation of a nasopharyngeal stent in patients with obstructive sleep-related breathing disorders.

The Journal of international medical research

Dellweg A, Kampmann M, Tschopp K

The Lonafarnib target, Rhes, is uniquely dysregulated in tauopathies: A human postmortem study.

Alzheimer's & Dementia

Alexander J. Ehrenberg, Kun Leng, Kaitlin Letourneau, Israel Hernandez, Caroline Lew, William W. Seeley, Salvatore Spina, Bruce L. Miller, Helmut Heinsen, Martin Kampmann, Kenneth S. Kosik, Lea T. Grinberg

Functional Multi-Omics Reveals Genetic and Pharmacologic Regulation of Surface CD38 in Multiple Myeloma.

Blood

Priya Choudhry, Olivia Gugliemini, Huimin Geng, Vishesh Sarin, Letitia Sarah, Yu-Hsiu T. Lin, Neha Paranjape, Poornima Ramkumar, Makeba Marcoulis, Donghui Wang, Paul Phojanakong, Veronica Steri, Byron Hann, Martin Kampmann, Arun P Wiita

BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2.

bioRxiv : the preprint server for biology

Tian R, Samelson AJ, Rezelj VV, Chen M, Ramadoss GN, Guo X, Kain AM, Tran QD, Lim SA, Lui I, Nunez J, Rockwood SJ, Liu N, Carlson-Stevermer J, Oki J, Maures T, Holden K, Weissman JS, Wells JA, Conklin B, Vignuzzi M, Kampmann M

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

eLife

Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J

Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis.

Nature neuroscience

Tian R, Abarientos A, Hong J, Hashemi SH, Yan R, Dräger N, Leng K, Nalls MA, Singleton AB, Xu K, Faghri F, Kampmann M

A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling.

The FASEB Journal

Khairunnisa Semesta, Ruilin Tian, Martin Kampmann, Mark Zastrow, Nikoleta Tsvetanova

Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing.

Cell

Nuñez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, Gilbert LA, Weissman JS

Patterns of neuronal Rhes as a novel hallmark of tauopathies.

Acta neuropathologica

Ehrenberg AJ, Leng K, Letourneau KN, Hernandez I, Lew C, Seeley WW, Spina S, Miller B, Heinsen H, Kampmann M, Kosik KS, Grinberg LT

Functional genomics screen identifies proteostasis targets that modulate prion protein (PrP) stability.

Cell stress & chaperones

Abrams J, Arhar T, Mok SA, Taylor IR, Kampmann M, Gestwicki JE

Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes.

The Journal of cell biology

Kanfer G, Sarraf SA, Maman Y, Baldwin H, Dominguez-Martin E, Johnson KR, Ward ME, Kampmann M, Lippincott-Schwartz J, Youle RJ

Molecular characterization of selectively vulnerable neurons in Alzheimer's disease.

Nature neuroscience

Leng K, Li E, Eser R, Piergies A, Sit R, Tan M, Neff N, Li SH, Rodriguez RD, Suemoto CK, Leite REP, Ehrenberg AJ, Pasqualucci CA, Seeley WW, Spina S, Heinsen H, Grinberg LT, Kampmann M

A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling.

PLoS genetics

Semesta KM, Tian R, Kampmann M, von Zastrow M, Tsvetanova NG

Defining the ATPome reveals cross-optimization of metabolic pathways.

Nature communications

Bennett NK, Nguyen MK, Darch MA, Nakaoka HJ, Cousineau D, Ten Hoeve J, Graeber TG, Schuelke M, Maltepe E, Kampmann M, Mendelsohn BA, Nakamura JL, Nakamura K

Robust Sequence Determinants of α-Synuclein Toxicity in Yeast Implicate Membrane Binding.

ACS chemical biology

Newberry RW, Arhar T, Costello J, Hartoularos GC, Maxwell AM, Naing ZZC, Pittman M, Reddy NR, Schwarz DMC, Wassarman DR, Wu TS, Barrero D, Caggiano C, Catching A, Cavazos TB, Estes LS, Faust B, Fink EA, Goldman MA, Gomez YK, Gordon MG, Gunsalus LM, Hoppe N, Jaime-Garza M, Johnson MC, Jones MG, Kung AF, Lopez KE, Lumpe J, Martyn C, McCarthy EE, Miller-Vedam LE, Navarro EJ, Palar A, Pellegrino J, Saylor W, Stephens CA, Strickland J, Torosyan H, Wankowicz SA, Wong DR, Wong G, Redding S, Chow ED, DeGrado WF, Kampmann M

CRISPR-based screens uncover determinants of immunotherapy response in multiple myeloma.

Blood advances

Ramkumar P, Abarientos AB, Tian R, Seyler M, Leong JT, Chen M, Choudhry P, Hechler T, Shah N, Wong SW, Martin TG, Wolf JL, Roybal KT, Pahl A, Taunton J, Wiita AP, Kampmann M

Pharmaceutical-Grade Rigosertib Is a Microtubule-Destabilizing Agent.

Molecular cell

Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS

A High-throughput CRISPR Interference Screen to Dissect Functional Regulators of GPCR/cAMP Signaling.

The FASEB Journal

Khairunnisa Mentari Semesta, Ruilin Tian, Martin Kampmann, Mark von Zastrow, Nikoleta G. Tsvetanova

LRP1 is a master regulator of tau uptake and spread.

Nature

Rauch JN, Luna G, Guzman E, Audouard M, Challis C, Sibih YE, Leshuk C, Hernandez I, Wegmann S, Hyman BT, Gradinaru V, Kampmann M, Kosik KS

Deep mutational scanning reveals the structural basis for α-synuclein activity.

Nature chemical biology

Newberry RW, Leong JT, Chow ED, Kampmann M, DeGrado WF

Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.

Nature

Guo X, Aviles G, Liu Y, Tian R, Unger BA, Lin YT, Wiita AP, Xu K, Correia MA, Kampmann M

Gene expression and cell identity controlled by anaphase-promoting complex.

Nature

Oh E, Mark KG, Mocciaro A, Watson ER, Prabu JR, Cha DD, Kampmann M, Gamarra N, Zhou CY, Rape M

Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation.

The Journal of biological chemistry

Chen JJ, Nathaniel DL, Raghavan P, Nelson M, Tian R, Tse E, Hong JY, See SK, Mok SA, Hein MY, Southworth DR, Grinberg LT, Gestwicki JE, Leonetti MD, Kampmann M

Quantitative Proteomics of Uukuniemi Virus-host Cell Interactions Reveals GBF1 as Proviral Host Factor for Phleboviruses.

Molecular & cellular proteomics : MCP

Uckeley ZM, Moeller R, Kühn LI, Nilsson E, Robens C, Lasswitz L, Lindqvist R, Lenman A, Passos V, Voss Y, Sommerauer C, Kampmann M, Goffinet C, Meissner F, Överby AK, Lozach PY, Gerold G

CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.

Neuron

Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M

Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether.

eLife

Torres SE, Gallagher CM, Plate L, Gupta M, Liem CR, Guo X, Tian R, Stroud RM, Kampmann M, Weissman JS, Walter P

Probing the Global Cellular Responses to Lipotoxicity Caused by Saturated Fatty Acids.

Molecular cell

Piccolis M, Bond LM, Kampmann M, Pulimeno P, Chitraju C, Jayson CBK, Vaites LP, Boland S, Lai ZW, Gabriel KR, Elliott SD, Paulo JA, Harper JW, Weissman JS, Walther TC, Farese RV

Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity.

Science (New York, N.Y.)

Zhang YJ, Guo L, Gonzales PK, Gendron TF, Wu Y, Jansen-West K, O'Raw AD, Pickles SR, Prudencio M, Carlomagno Y, Gachechiladze MA, Ludwig C, Tian R, Chew J, DeTure M, Lin WL, Tong J, Daughrity LM, Yue M, Song Y, Andersen JW, Castanedes-Casey M, Kurti A, Datta A, Antognetti G, McCampbell A, Rademakers R, Oskarsson B, Dickson DW, Kampmann M, Ward ME, Fryer JD, Link CD, Shorter J, Petrucelli L

Relapse-associated AURKB blunts the glucocorticoid sensitivity of B cell acute lymphoblastic leukemia.

Proceedings of the National Academy of Sciences of the United States of America

Poulard C, Kim HN, Fang M, Kruth K, Gagnieux C, Gerke DS, Bhojwani D, Kim YM, Kampmann M, Stallcup MR, Pufall MA

Identifying Factors in Multiple Myeloma Controlling Response to B-Cell Maturation Antigen (BCMA)-Targeted Immunotherapy Using CRISPR-Based Functional Genomics.

Blood

Poornima Ramkumar, Jaime Leong, Meghan Seyler, Stratton J Georgoulis, Axel Hyrenius Wittsten, Priya Choudhry, Kole Roybal, Arun P Wiita, Martin Kampmann

A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure.

PLoS biology

Mendelsohn BA, Bennett NK, Darch MA, Yu K, Nguyen MK, Pucciarelli D, Nelson M, Horlbeck MA, Gilbert LA, Hyun W, Kampmann M, Nakamura JL, Nakamura K

The Psychiatric Cell Map Initiative: A Convergent Systems Biological Approach to Illuminating Key Molecular Pathways in Neuropsychiatric Disorders.

Cell

Willsey AJ, Morris MT, Wang S, Willsey HR, Sun N, Teerikorpi N, Baum TB, Cagney G, Bender KJ, Desai TA, Srivastava D, Davis GW, Doudna J, Chang E, Sohal V, Lowenstein DH, Li H, Agard D, Keiser MJ, Shoichet B, von Zastrow M, Mucke L, Finkbeiner S, Gan L, Sestan N, Ward ME, Huttenhain R, Nowakowski TJ, Bellen HJ, Frank LM, Khokha MK, Lifton RP, Kampmann M, Ideker T, State MW, Krogan NJ

Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance.

Biology open

Mavor D, Barlow KA, Asarnow D, Birman Y, Britain D, Chen W, Green EM, Kenner LR, Mensa B, Morinishi LS, Nelson CA, Poss EM, Suresh P, Tian R, Arhar T, Ary BE, Bauer DP, Bergman ID, Brunetti RM, Chio CM, Dai SA, Dickinson MS, Elledge SK, Helsell CVM, Hendel NL, Kang E, Kern N, Khoroshkin MS, Kirkemo LL, Lewis GR, Lou K, Marin WM, Maxwell AM, McTigue PF, Myers-Turnbull D, Nagy TL, Natale AM, Oltion K, Pourmal S, Reder GK, Rettko NJ, Rohweder PJ, Schwarz DMC, Tan SK, Thomas PV, Tibble RW, Town JP, Tsai MK, Ugur FS, Wassarman DR, Wolff AM, Wu TS, Bogdanoff D, Li J, Thorn KS, O'Conchúir S, Swaney DL, Chow ED, Madhani HD, Redding S, Bolon DN, Kortemme T, DeRisi JL, Kampmann M, Fraser JS

Mapping the Genetic Landscape of Human Cells.

Cell

Horlbeck MA, Xu A, Wang M, Bennett NK, Park CY, Bogdanoff D, Adamson B, Chow ED, Kampmann M, Peterson TR, Nakamura K, Fischbach MA, Weissman JS, Gilbert LA

Tau Internalization is Regulated by 6-O Sulfation on Heparan Sulfate Proteoglycans (HSPGs).

Scientific reports

Rauch JN, Chen JJ, Sorum AW, Miller GM, Sharf T, See SK, Hsieh-Wilson LC, Kampmann M, Kosik KS

CRISPR-based genetic interaction maps inform therapeutic strategies in cancer.

Translational cancer research

Ramkumar P, Kampmann M

Dual gene activation and knockout screen reveals directional dependencies in genetic networks.

Nature biotechnology

Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT

Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent.

Molecular cell

Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS

Abstract A30: Identifying microenvironment-dependent vulnerabilities in multiple myeloma using CRISPRi.

Finding Synthetic Lethal Interactions through Functional Genomics

Poornima Ramkumar, Jaime Tawney, Diego Acosta-Alvear, Martin Kampmann

A CRISPR Approach to Neurodegenerative Diseases.

Trends in molecular medicine

Kampmann M

Suppression of B-cell development genes is key to glucocorticoid efficacy in treatment of acute lymphoblastic leukemia.

Blood

Kruth KA, Fang M, Shelton DN, Abu-Halawa O, Mahling R, Yang H, Weissman JS, Loh ML, Müschen M, Tasian SK, Bassik MC, Kampmann M, Pufall MA

Inhibiting pathways involved in B-cell development enhances sensitivity of B-cell acute lymphoblastic leukemia to glucocorticoids.

The FASEB Journal

Miles A Pufall, Karina Kruth, Mimi A Fang, Dawne Shelton, Ossama Abu-Halawa, Sarah K Tasian, Martin Kampmann

The mTOR Complex Controls HIV Latency.

Cell host & microbe

Besnard E, Hakre S, Kampmann M, Lim HW, Hosmane NN, Martin A, Bassik MC, Verschueren E, Battivelli E, Chan J, Svensson JP, Gramatica A, Conrad RJ, Ott M, Greene WC, Krogan NJ, Siliciano RF, Weissman JS, Verdin E

Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.

eLife

Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS

Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification.

Nature chemical biology

Deans RM, Morgens DW, Ökesli A, Pillay S, Horlbeck MA, Kampmann M, Gilbert LA, Li A, Mateo R, Smith M, Glenn JS, Carette JE, Khosla C, Bassik MC

Targeting the AAA ATPase p97 as an Approach to Treat Cancer through Disruption of Protein Homeostasis.

Cancer cell

Anderson DJ, Le Moigne R, Djakovic S, Kumar B, Rice J, Wong S, Wang J, Yao B, Valle E, Kiss von Soly S, Madriaga A, Soriano F, Menon MK, Wu ZY, Kampmann M, Chen Y, Weissman JS, Aftab BT, Yakes FM, Shawver L, Zhou HJ, Wustrow D, Rolfe M

Abstract A43: Opposing roles of the 19S regulatory- and 20S core-proteasomal subunits in controlling sensitivity of multiple myeloma cells to proteasome inhibition.

Myeloma

Diego Acosta-Alvear, Martin Kampmann, Min Y. Cho, Thomas Wild, Chunaram Choudhary, Marc A. Shuman, Jonathan S. Weissman, Peter Walter

Paradoxical resistance of multiple myeloma to proteasome inhibitors by decreased levels of 19S proteasomal subunits.

eLife

Acosta-Alvear D, Cho MY, Wild T, Buchholz TJ, Lerner AG, Simakova O, Hahn J, Korde N, Landgren O, Maric I, Choudhary C, Walter P, Weissman JS, Kampmann M

Next-generation libraries for robust RNA interference-based genome-wide screens.

Proceedings of the National Academy of Sciences of the United States of America

Kampmann M, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

eLife

Sidrauski C, Tsai JC, Kampmann M, Hearn BR, Vedantham P, Jaishankar P, Sokabe M, Mendez AS, Newton BW, Tang EL, Verschueren E, Johnson JR, Krogan NJ, Fraser CS, Weissman JS, Renslo AR, Walter P

Validation of the Hsp70-Bag3 protein-protein interaction as a potential therapeutic target in cancer.

Molecular cancer therapeutics

Li X, Colvin T, Rauch JN, Acosta-Alvear D, Kampmann M, Dunyak B, Hann B, Aftab BT, Murnane M, Cho M, Walter P, Weissman JS, Sherman MY, Gestwicki JE

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.

Cell

Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS

Weak base pairing in both seed and 3' regions reduces RNAi off-targets and enhances si/shRNA designs.

Nucleic acids research

Gu S, Zhang Y, Jin L, Huang Y, Zhang F, Bassik MC, Kampmann M, Kay MA

Unraveling the mechanism of cell death induced by chemical fibrils.

Nature chemical biology

Julien O, Kampmann M, Bassik MC, Zorn JA, Venditto VJ, Shimbo K, Agard NJ, Shimada K, Rheingold AL, Stockwell BR, Weissman JS, Wells JA

Unraveling the Mechanism of Cell Death Induced by Chemical and Protein Fibrils.

Biophysical journal

Olivier Julien, Martin Kampmann, Michael C. Bassik, Vincent J. Venditto, Julie A. Zorn, Arnold L. Rheingold, Jonathan S. Weissman, James A. Wells

Next-Generation NAMPT Inhibitors For ALL Identified By Sequential High-Throughput Phenotypic Chemical and Functional Genomic Screens.

Blood

Michael C. Wei, Christina J. Matheny, Michael C. Bassik, Alicia J. Donnelly, Martin Kampmann, Masayuki Iwasaki, Obdulio Piloto, David E. Solow-Cordero, Donna Bouley, Rachel E. Rau, Patrick Brown, Michael T. McManus, Jonathan S. Weissman, Michael L. Cleary

Next-generation NAMPT inhibitors identified by sequential high-throughput phenotypic chemical and functional genomic screens.

Chemistry & biology

Matheny CJ, Wei MC, Bassik MC, Donnelly AJ, Kampmann M, Iwasaki M, Piloto O, Solow-Cordero DE, Bouley DM, Rau R, Brown P, McManus MT, Weissman JS, Cleary ML

Abstract C01: Systematic blueprinting of genetic vulnerabilities using ultra-complex shRNA libraries identifies genes synergizing with proteasome inhibition or blockade of IRE1 in multiple myeloma cells.

Signaling and Drug Resistance

Diego Acosta-Alvear, Martin Kampmann, Michael C. Bassik, Crystal P. Lee, Min Y. Cho, Blake T. Aftab, Xiaokai Li, Jason E. Gestwicki, Marc A. Shuman, Jonathan S. Weissman, Peter Walter

Abstract PR07: Systematic genetic interaction maps reveal rewiring of the stress response network and resulting vulnerabilities in leukemia and multiple myeloma cells.

Interpreting the Cancer Genome

Martin Kampmann, Diego Acosta-Alvear, Min Cho, Yuwen Chen, Xiaokai Li, Luke Gilbert, Blake T. Aftab, Jason Gestwicki, Peter Walter, Jonathan S. Weissman

Integrated platform for genome-wide screening and construction of high-density genetic interaction maps in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America

Kampmann M, Bassik MC, Weissman JS

Abstract A08: Systematic identification of genetic vulnerabilities synergizing with proteasome and IRE1 inhibition in multiple myeloma cells using ultra-complex shRNA libraries.

Genome Scale Synthetic Lethal Screens in Model Organisms

Diego Acosta-Alvear, Martin Kampmann, Michael C. Bassik, Crystal P. Lee, Marc A. Shuman, Jonathan S. Weissman, Peter Walter

Abstract PR06: Systematic genetic interaction maps reveal synthetic-lethal vulnerabilities in leukemia and multiple myeloma.

Systemic Functional Genomics

Martin Kampmann, Diego Acosta-Alvear, Michael C. Bassik, Yuwen Chen, Peter Walter, Jonathan S. Weissman

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.

Cell

Bassik MC, Kampmann M, Lebbink RJ, Wang S, Hein MY, Poser I, Weibezahn J, Horlbeck MA, Chen S, Mann M, Hyman AA, Leproust EM, McManus MT, Weissman JS

Conserved spatial organization of FG domains in the nuclear pore complex.

Biophysical journal

Atkinson CE, Mattheyses AL, Kampmann M, Simon SM

Effect of FG Motifs on Ordering Proteins within the Nuclear Pore Complex.

Biophysical journal

Claire E. Atkinson, Alexa L. Mattheyses, Martin Kampmann, Sanford M. Simon

Knocking out the door to tunicamycin entry.

Proceedings of the National Academy of Sciences of the United States of America

Bassik MC, Kampmann M

Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy.

Nature structural & molecular biology

Kampmann M, Atkinson CE, Mattheyses AL, Simon SM

Protein Domain Organization in the Nuclear Pore Complex Studied by Fluorescence Anisotropy.

Biophysical journal

Alexa L. Mattheyses, Martin Kampmann, Claire E. Atkinson, Sanford M. Simon

The Structure of the Nuclear Pore Complex Studied by Fluorescence Anisotropy.

Biophysical journal

Claire E. Atkinson, Martin Kampmann, Alexa L. Mattheyses, Sanford M. Simon

Fluorescence anisotropy reveals order and disorder of protein domains in the nuclear pore complex.

Biophysical journal

Mattheyses AL, Kampmann M, Atkinson CE, Simon SM

Use of RNA tertiary interaction modules for the crystallisation of the spliceosomal snRNP core domain.

Journal of molecular biology

Leung AK, Kambach C, Kondo Y, Kampmann M, Jinek M, Nagai K

Biochemistry. Nascent proteins caught in the act.

Science (New York, N.Y.)

Kampmann M, Blobel G

Structure of a trimeric nucleoporin complex reveals alternate oligomerization states.

Proceedings of the National Academy of Sciences of the United States of America

Nagy V, Hsia KC, Debler EW, Kampmann M, Davenport AM, Blobel G, Hoelz A